Acute Phase ELISA Kits

Acute phase protein levels typically increase in blood as a result of inflammation, tissue injury, or disease and can often be used as surrogate biomarkers of disease progression. Importantly, acute phase biomarkers differ with regard to their response in different species. C-reactive protein (CRP)  for example can increase as much as 1000-fold in humans but increases by only 50% in rats. In contrast, alpha-2-macroglobulin increases ~100-fold in rats but does not increase at all in humans. Please contact us if you would like advice regarding biomarker selection. We currently manufacture ELISA kits for measurement of acute phase proteins including CRP, haptoglobin, hemopexin, alpha-1 acid glycoprotein, clusterin, alpha-2-macroglobulin, and serum amyloid A (SAA) from species including mouse, rat, rabbit, dog, pig, cow, chicken, and monkey.

Literature references that cite the use of our kits are listed at the bottom of this page.

Click on the “Insert” button for kit instructions

Orders may be placed by phone, fax, email, or via our online shop.

Discounts: 2-3 kits, 10%; >3 kits, 15%

The figure below illustrates the levels of four acute phase proteins (a-1-acid glycoprotein, ceruloplasmin, CRP, and haptoglobin) determined in serum from normal and diseased (adjuvant induced arthritis) rats using ELISA kits manufactured by Life Diagnostics, Inc.

Literature References that cite the use of LDI acute phase protein ELISA kits

Bovine Haptoglobin

1. Del Campo M, et.al. Effect of different feeding strategies on animal welfare and meat quality in Uruguayan steers. Meat Science 80(3): 753-760 (2008)
2. Kim M-H, The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. J. Vet. Sc. 12(2): 151-157 (2011)
3. Kwon IH, et. al. Effects of fermented soybean meal on immune response of weaned calves with experimentally induced lipopolysaccharide challenge. Asian-Aust. J. Anim. Sc. 24(7): 957-964 (2011)

Chicken alpha-1-acid glycoprotein

1. Lee KW, et. al. Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens. Poultry Science. 89: 203-216 (2010)
2. Li G, et. al. Immunopathology and cytokine responses in commercial broiler chickens with gangrenous dermatitis. Avian Pathology. 39(4): 255-264 (2010)

Dog CRP

1. Kjelgaard-Hansen M, et.al. Measurement of serum interleukin-10 in the dog. The Veterinary Journal. 173(2) 361-365 (2007)

Monkey Acute Phase Proteins

1. Ossetrova NI, et. al. The use of discriminant analysis for evaluation of early-response multiple biomarkers of radiation exposure using non-human primate 6-Gy whole body radiation model. Radiation Measurements. 42: 1158-1163 (2007) – CRP
2. Blackwood RS, et. al. Effects of the Macrolide drug Tylosin on Chronic Diarrhea in Rhesus Macaques (Macaca mullata). Comparative Medicine. 58(1): 81-87 (2008) – CRP
3. Blakely WF, et.al. Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications. Health Phys. 98(2): 153-159 (2010) – CRP
4. Ossetrova NI, et. al. C-reactive protein and serum amyloid-A as early-phase and prognostic indicators of acute radiation exposure in nonhuman primate total-body irradiation model. Radiation Measurements. 46: 1019-1024 (2011) – CRP & SAA

Mouse Acute Phase Proteins

1. Ito R, et. al. Interferon-gamma is causatively involved in experimental inflammatory bowel disease in mice. Clinical and Experimental Immunology. 146: 330-338 (2006) – Haptoglobin
2. Bishop DF, et. al. Uroporphyrinogen III synthase Knock-In mice have the human congenital erythropoietic porphyria phenotype, including the characteristic light-Induced cutaneous lesions. Am J Hum Genet. 78(4): 645-658 (2006) – Haptoglobin
3. Liu M, et al. Acute kidney injury leads to brain inflammation and functional changes. J Am Soc Neprol. 19(7): 1360-1370 (2008) - CRP
4. Chung M-C, et.al. Activation of plasminogen activator inhibitor implicates protease InhA in the acute-phase response to Bacillus anthracis infection. J Med Microbiol. 58: 737-744 (2009) – CRP, Haptoglobin, Hemopexin & SAA
5. Bem RA, et. al. Mechanical ventilation enhances lung inflammation and caspase activity in a model of mouse pneumovirus infection. Am J Physiol Lung Cell Mol Physiol 296: L46–L56 (2009) - Macroglobulin
6. Hod EA, et. al. Transfusion of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammation. Blood 115(21): 4284-4292 (2010) – SAA
7. Bem RA, et. al. Granzyme A- and B-cluster deficiency delays acute lung injury in pneumovirus-infected mice. The Journal of Immunology. 184: 931-938 (2010) – Macroglobulin
8. Teupser D, et.al. No reduction of atherosclerosis in C-reactive protein (CRP)-deficient mice. J Biol Chem. 286(8): 6272-6279 (2011) – CRP
9. Cray C, et.al. Quantitation of acute phase proteins and protein electrophoresis in monitoring the acute inflammatory process in experimentally and naturally infected mice.  Comparative Medicine. 60(4): 263-371 (2010 – CRP, Haptoglobin & SAA
10. Higashisaka K, et.al. Acute phase proteins as biomarkers for predicting the exposure and toxicity of nanomaterials. Biomaterials. 32: 3-9 (2011) - CRP, Haptoglobin & SAA
11. Lee KH, et.al. Effects of Lonicera japonica Thunb. On dextran sulfate sodium-induces experimental colitis in mice. J Medicinal Plants Research. 5(22) 5437-5443 (2011) – SAA
12. Stanton MC, et. Al. Inflammatory signals shift from adipose to liver during high fat feeding and influence the development of steatohepatitis in mice. http://www.journal-inflammation.com/content/8/1/8 (2011) – SAA
13. Matsumoto M, et. al. Longevity in mice is promoted by probiotic-induced suppression of colonic senescence dependent on upregulation of gut bacterial polyamine production. PLoS One. 2011; 6(8): e23652. 10.1371/journal.pone.0023652 (2011) – Haptoglobin

     

Pig Haptoglobin

1. Patterson JK, et. al. Dietary Inulin Supplementation does not promote colonic iron absorption in a porcine model. J. Agric. Food Chem. 57: 5250-5256 (2009)

Rabbit CRP

1. Qian Z, et. al. Targeting vascular injury using hantavirus-pseudotyped lentiviral vectors. Molecular Therapy 13(4): 694-704 (2006)
2. Cenesiz S, et.al. Effects of fluoride on C-reactive protein, adenosine deaminase, and ceruloplasmin in rabbit sera. Research Report Fluoride 41(1): 52-56 (2008) http://www.fluorideresearch.org/411/files/FJ2008_v41_n1_p052-056.pdf

Rat Acute Phase Proteins

1. Bledsoe SL, et.al. Pravastatin and clpidogrel combined inhibit intimal htperplasia in a rat carotid endarterectomy model. Vasc Endovasc Surg. 40: 49-57 (2006 ) – CRP
2. Schouw Nielsem S, et. al. Synthesis of acute phase proteins in rats with cirrhosis exposed to lipopolysaccharide. http://www.comparative-hepatology.com/content/5/1/3  (2006) – Alpha-1-acid glycoprotein, Haptoglobin
3. Aller M-A, et. al. Plasma redox status is impaired in the portacaval shunted rat – the risk for reduced antioxidant ability. http://www.comparative-hepatology.com/content/7/1/1 (2008) – Thiostatin
4. Kiraly MA, et. al. Exercise maintains euglycemia in association with decreased activation of c-jun NH₂-terminal kinase and serine phosphorylation of IRS-1 in the liver of ZDF rats. Am J Physiol Endocrinol Metab 289: E671-E682 (2010) – Haptoglobin
5. Ansede JH, et. al. Characterization of sandwich-cultured hepatocytes as an in vitro model to assess the hepatobiliary disposition of copper. Am Soc Pharm Exp Ther. 37(5): 969-976 (2009) – Ceruloplasmin
6. Gillet A, et.al. TNF production in macrophages is genetically determined and regulates inflammatory disease in rats. J Immunol. 185: 442-450 (2010) – Alpha-1-acid glycoprotein